Metadata
The metadata format
The metadata file in its simplest form is a two column table (.csv format). The first column has to be named SAMPLE and contains in each row the sample id matching exactly the name used for the input file columns. In case of gel images as input, the first n entries will be used, where n equals the number of lanes in the gel.
SAMPLE |
YOUR_VARIABLE_1 |
YOUR_VARIABLE_2 |
|---|---|---|
Sample_1 |
HEALTHY |
FEMALE |
Sample_2 |
HEALTHY |
FEMALE |
… |
… |
… |
Sample_100 |
DISEASED |
MALE |
Adding more metadata
To add more metadata, simply add additional columns into the metadata file.
SAMPLE |
CONDITION |
EXPERIMENT |
SEX |
AGE |
INFECTION |
|---|---|---|---|---|---|
Sample_1 |
Control |
1 |
M |
10 |
Yes |
Sample_2 |
Control |
1 |
F |
24 |
Yes |
Sample_3 |
Control |
1 |
M |
7 |
Yes |
Sample_4 |
Control |
1 |
M |
87 |
Yes |
Sample_5 |
Treat |
1 |
F |
34 |
Yes |
Sample_6 |
Treat |
2 |
F |
56 |
No |
Sample_7 |
Treat |
2 |
F |
66 |
No |
Sample_8 |
Treat |
2 |
F |
25 |
No |
Adding metadata for gel images
Since gel images do not contain lane names, the samples will be automatically named by lane number (e.g. 1,2,3…). To add metadata for such samples, the row number in the metadata file will dictate the lane number the metadata is applied to.